Figure 1: Expression and localization of PfFNT in Xenopus laevis oocytes.
From: A lactate and formate transporter in the intraerythrocytic malaria parasite, Plasmodium falciparum
(a) Western blots of membranes from oocytes expressing C-terminal tagged HA-PfFNT, probed with an anti-HA antibody, show a band close to the predicted size of 34 kDa. The band is absent from membranes from non-injected (ni) oocytes. (b) Immunofluorescence images of oocytes injected with cRNA encoding C-terminal tagged HA-PfFNT show a clear band of fluorescence (corresponding to the plasma membrane) outside the pigment layer. The band is not seen in non-injected oocytes. Scale bar, 100 μm. In radiolabel uptake experiments, oocytes expressing the HA-tagged form of PfFNT accumulated [3H]formate to levels above those measured in non-injected oocytes, consistent with the tagged protein being functional.
