Figure 5: Localization of HA-PfFNT in 3D7 P. falciparum trophozoites.
From: A lactate and formate transporter in the intraerythrocytic malaria parasite, Plasmodium falciparum
(a) Western blot analysis of membrane preparations from P. falciparum trophozoites expressing either N-terminal or C-terminal tagged HA-PfFNT (lanes N and C, respectively), with a protein corresponding to the predicted size of 34 kDa identified in both cases. (b) An image of parasitized erythrocytes expressing C-terminal HA-tagged PfFNT, showing immunofluorescence both at the surface of the intraerythrocytic parasite and in the region surrounding the hemozoin crystals. Similar results were obtained with N-terminal HA-tagged PfFNT (not shown). (c) Co-localization of C-terminal tagged HA-PfFNT with the parasite’s plasma membrane nucleoside/nucleobase transporter PfENT1. Scale bar, 5 μm.
