Figure 2: Beneficial effects of fibre in DSS colitis are mediated through GPR43 and GPR109A, and expressed in non-haematopoietic cells. | Nature Communications

Figure 2: Beneficial effects of fibre in DSS colitis are mediated through GPR43 and GPR109A, and expressed in non-haematopoietic cells.

From: Metabolite-sensing receptors GPR43 and GPR109A facilitate dietary fibre-induced gut homeostasis through regulation of the inflammasome

Figure 2

Clinical scores measured in WT (a,d), in Gpr43−/− mice (a) and in Gpr109A−/− mice (d) fed on zero-fibre (ZF) or on high-fibre (HF) diets for 7 days prior and during the 6 days treatment with 2% DSS. (b,f) Colon length measured at day 6 in DSS-treated Gpr43−/− mice (b) or Gpr109A−/− mice (f) versus WT mice fed on ZF or HF diets. (c,e) Histological score and representative haematoxylin and eosin (H&E)-stained colonic sections of HF-DSS- and ZF-DSS-fed Gpr43−/− (c) or Gpr109A−/− (e) mice treated with DSS (n=6 mice per group, repeated twice); scale bars, 200 μm. (g) Clinical scores assessed in irradiated Gpr43−/− mice reconstituted with Gpr43−/− bone marrow (BM; Gpr43−/− BM into Gpr43−/−) fed on HF diet (n=6) or with WT BM (BL6BM into Gpr43−/−) fed on HF (n=5) or on ZF (n=5) diets, in irradiated WT mice reconstituted with WT BM cells (BL6BM into BL6) fed on HF diet (n=5) or in irradiated WT mice reconstituted with Gpr43−/− BM (Gpr43−/− BM into BL6) fed on HF (n=10) or on ZF (n=10) diets. Mice were fed on diets for a week prior and during treatment with 2% DSS for 6 days. (h) Representative H&E-stained colonic sections of HF-DSS and ZF-DSS-fed irradiated WT mice reconstituted with Gpr43−/− BM cells (Gpr43−/−BM into BL6) and in irradiated Gpr43−/− mice reconstituted with WT BM cells (BL6BM into Gpr43−/−); scale bar, 200 μm. The results are shown as mean±s.e.m. of at least n=6 mice per group repeated twice. With *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 as group effect as determined by two-way analysis of variance, #P<0.05 as comparison between WT HF-DSS and Gpr43−/− HF-DSS, $P<0.05, $$P<0.01 as differences between Gpr43−/− HF-DSS versus ZF-DSS determined by Tukey’s multiple analysis (a), #P<0.05, ##P<0.01, ####P<0.0001 as comparison between WT HF-DSS and Gpr109A−/− HF-DSS by Sidak’s multiple comparison analysis (d), #P<0.05, ##P<0.01 and ###P<0.001 as comparison between BL6BM into Gpr43−/− HF versus BL6BM into BL6 HF determined by Tukey’s multiple comparison analysis (g) and **P<0.01 by t-test.

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