Figure 5: Expression of NLRP3 in non-haematopoietic cells is necessary for the beneficial effects of high-fibre feeding in DSS colitis.

Clinical score (a), colon length (b), histological score (c) and representative haematoxylin and eosin-stained colonic sections (d) were determined in irradiated WT mice reconstituted with Nlrp3^−/− bone marrow (BM) cells (Nlrp3^−/−BM into BL6) fed on zero fibre (ZF; n=9) and on high-fibre diet (HF; n=9), in irradiated Nlrp3^−/−mice reconstituted with WT BM cells (BL6BM into Nlrp3^−/−) fed on ZF (n=5) or on HF (n=5), in irradiated WT mice reconstituted with WT BM cells (BL6BM into BL6) fed on HF (n=5), and in irradiated Nlrp3^−/− mice reconstituted with Nlrp3^−/− BM cells (Nlrp3^−/−BM into Nlrp3^−/−) fed on HF (n=6) before and during treatment with 2% DSS or water. The results are shown as mean±s.e.m., scale bar=200 μm. With *P<0.05 and ****P<0.0001 as interaction of group effects as determined by analysis of variance, ^$$$P<0.001 comparing BL6BM into Nlrp3^−/− HF versus BL6BM into BL6 HF, ^####P<0.0001 comparing Nlrp3^−/−BM into BL6 HF versus Nlrp3^−/−BM into Nlrp3^−/− HF by Tukey’s multiple comparison analysis and *P<0.05 **P<0.01, ***P<0.001 and ****P<0.0001 determined by t-test.