Figure 8: Effects of depletion of XPF on replication fork progression in Mus81−/− cells. | Nature Communications

Figure 8: Effects of depletion of XPF on replication fork progression in Mus81−/− cells.

From: The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage

Figure 8

HCT116 and Mus81−/− cells were transfected with siRNA directed against XPF or control siRNA. (a) Western blot analysis revealed an efficient knockdown of XPF protein via siRNA treatment. (b,c) Cells were sequentially pulsed with IdU and CldU for 20 min, respectively, before harvesting for DNA combing. Scatter plot was used to show the distribution of replication fork speed (b) and inter-origin distance (c). Median with interquartile range was shown for the scatter plot. Mann–Whitney test was used for the statistical analysis. A few extreme numbers (>4.5 kb min−1 for fork speed, >400 kb for origin distance) were not shown for a better resolution of the scatter plot. Statistical analyses were shown in Supplementary Table 4.

Back to article page