Figure 4: SALL4 recruits the polycomb complexes PRC1 and PRC2 to the Sox-2 locus and the PRC1 complex to the Sox-17 locus.
From: A cohesin–OCT4 complex mediates Sox enhancers to prime an early embryonic lineage
Sequential ChIP–PCR anti-SUZ12 or anti-BMI1 and then anti-SALL4 were performed from chromatin of control ZHBTc6 (+tetracyclin) ESCs (CTRL) or OCT4-overexpressing (ZHBTc6−tetracyclin) mesendodermal cells (Oct4OE). Both Sox-2C (a) and Sox-17 (b) enhancers and promoters were interrogated. Sox2B and Sox17R2 regions were used as negative controls. (c) ChIP anti-SUZ12 (left panel) and anti-BMI (right panel) were performed from chromatin of control ZHBTc6 (+tetracyclin) ESCs (CTRL) or OCT4-overexpressing (ZHBTc6−tetracyclin) mesendodermal cells (Oct4OE). Both Sox-2C and Sox-17 enhancers were interrogated. Data are expressed as mean±s.e.m., from three experiments, *Student’s t-test, P≤0.01.
