Figure 3: Quantifying changes in arginine methylation occupancy during primary T cell differentiation. | Nature Communications

Figure 3: Quantifying changes in arginine methylation occupancy during primary T cell differentiation.

From: Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling

Figure 3

(a) CD4+ T cells are isolated from human blood, a portion is collected directly (unstimulated). The remainder of the cells are stimulated with anti-CD3, anti-CD28 antibodies and IL-2. Cells proliferate and are stable isotope labelled for 7–9 days. Purified arginine-methylated peptides are identified and quantified. Non-methylated peptides found in the supernatant are fractionated and also identified and quantified. Quantification information is combined to calculate changes in arginine methylation occupancy. (b) Differential arginine methylation during T-cell differentiation. Thirty-seven per cent (134/365) of arginine-methylated peptides significantly increase (red) or decrease (green) in Heavy/Light (H/L) ratio during stimulation of T cells (left). After normalizing for changes in corresponding protein levels, >10% (27/282) of arginine-methylated peptides significantly increase (red) or decrease (green) in occupancy (right). (c) Interaction network of proteins showing changes in arginine methylation occupancy during T-cell stimulation. Proteins are coloured according to the change in arginine methylation occupancy, where a protein contains multiple sites, each is coloured individually.

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