Figure 3: Neuronal HttQ91 and glial HttQ25 aggregates associate with cytoplasmic markers.
From: Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain
(a–c) Co-localization of HttQ91 or HttQ25 aggregates with the endogenous cytoplasmic markers Hsp70/Hsc70, Hsp90 and ubiquitin. Single 1.0-μm optical Z-slices from the DA1 glomerulus of 7-day-old adult flies expressing HttQ91-mCherry in DA1 ORNs and HttQ25-YFP in glia and immunostained with antibodies against Hsp70/Hsc70 (a), Hsp90 (b) and ubiquitin (c) are shown. Unfilled arrowheads indicate HttQ91 aggregates that lack associated HttQ25 signal, and filled arrows indicate induced HttQ25 aggregates in glia. Scale bars, 5 μm. (d) Quantification of the % co-localization of each aggregate subtype with the endogenous cytoplasmic markers (n≥4, ±s.e.m., *P<0.05, **P<0.01, ***P<0.001, one-way analysis of variance followed by Tukey’s post hoc test).
