Figure 4: Draper is required for nucleation of glial HttQ25 aggregation by neuronal HttQ91 aggregates. | Nature Communications

Figure 4: Draper is required for nucleation of glial HttQ25 aggregation by neuronal HttQ91 aggregates.

From: Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain

Figure 4

(a) Draper is essential for transfer of HttQ91 aggregates from DA1 ORN axons to the glial cytoplasm. Maximum intensity Z-projections of the DA1 glomerulus from 7-day-old adult fly brains expressing HttQ91-mCherry in DA1 ORNs and HttQ25-YFP in glia, either without (control) or with depletion of Draper expression using two independent RNAi lines (DraperRNAi1 and DraperRNAi2) or the drprΔ5 null mutation (draper−/−). Scale bars, 5 μm. (b) Quantification of the number of induced HttQ25 aggregates detected in the antennal lobe of 7-day-old adult flies as in a (n≥8, ±s.e.m., ***P<0.0005, one-way analysis of variance followed by Tukey’s post hoc test).

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