Figure 5: Nucleation of glial HttQ25 aggregation by neuronal HttQ91 aggregates requires phagocytic engulfment genes. | Nature Communications

Figure 5: Nucleation of glial HttQ25 aggregation by neuronal HttQ91 aggregates requires phagocytic engulfment genes.

From: Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain

Figure 5

(a–k) Shark, Rac1, Crk and dCed12 mediate entry of HttQ91 aggregates into the glial cytoplasm to induce aggregation of HttQ25. Maximum intensity Z-projections of the DA1 glomerulus from 6- to 8-day-old adult fly brains expressing HttQ91-mCherry in DA1 ORNs and HttQ25-YFP in glia, without (a) or with (b–i) glial expression of double-stranded RNAs targeting shark (b), Rac1 (c), Crk (d,e), dCed12 (f) or bsk (g–i). Scale bars, 5 μm. (j,k) Average numbers of HttQ91 aggregates (j) or induced HttQ25 aggregates (k) detected in the antennal lobe of 6– to 8-day-old adult fly brains as shown in a–i (n≥8, ±s.e.m., *P<0.05, **P<0.005, ***P<0.0005, one-way analysis of variance followed by Tukey’s post hoc test).

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