Figure 4: In vitro stability of the CDK1–cyclin B and CDK2–cyclin A complexes.
From: CDK1 structures reveal conserved and unique features of the essential cell cycle CDK
(a–d) Isothermal titration calorimetry thermograms to assess the formation of CDK2–cyclin A (a,d) and CDK1–cyclin B (b,c) complexes under conditions of lower salt, lower pH (40 mM HEPES, pH 7.4, 200 mM NaCl, 0.01% monothioglycerol) (a,b) and higher salt, higher pH (50 mM HEPES, pH 8.0, 500 mM NaCl, 0.01% monothioglycerol) (c,d). (e) Comparison of the thermal stability of the phosphorylated CDK1–cyclin B and CDK2–cyclin A complexes as assessed by differential scanning fluorimetry. (f) Comparison of the thermal stability of phosphorylated CDK1–cyclin B after either treatment with λ-phosphatase, or mock-treatment under identical conditions. (e,f) Representative melting curves are shown from two replicates.
