Figure 3: Decreased carcinoma cell proliferation in mammary tumours with reduced matriptase.
(a) Representative pictures of mammary tumours from PymT-Mat+ (top) and PymT-Mathypo (bottom) stained with an anti-BrdU antibody. Mice were injected with BrdU 2 h before euthanasia. (b) Quantification of carcinoma cell proliferation in 127-day-old mice. Data are presented as the mean relative number of cells with positive nuclear BrdU staining (arrowheads) in tumours from PymT-Mat+ (N=4, black bar), and littermate PymT-Mathypo mice (N=3, white bar). (c) Quantification of carcinoma cell proliferation in 145-day-old mice. Data are presented as the mean relative number of cells with positive nuclear Ki67 staining in tumours from PymT-Mat+ (N=4, black bar), and littermate PymT-Mathypo mice (N=4, white bar). (d) Tumour-associated macrophages in 145-day-old mice. Macrophages were visualized using a rabbit anti-F4/80 antibody (arrowheads). (e) Apoptotic carcinoma cells detected by TUNEL staining in 145-day-old mice (arrowheads). (f) Quantification of tumour-associated macrophages. Data are presented as the mean relative area with positive F4/80 staining in tumours from PymT-Mat+ (N=4, black bar), and littermate PymT-Mathypo mice (N=3, white bar). (g) Quantification of cells with TUNEL-positive nuclear staining in tumours from PymT-Mat+ (N=4, black bars), and littermate PymT-Mathypo mice (N=3, white bars). All panels: Error bars represent s.d., *P<0.001, Mann–Whitney U-test. Scale bars, 50 μm.
