Figure 7: Nedd4 accelerates zebrafish embryonic growth through Irs-2.
From: Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity
(a) Nedd4-Venus mRNA or Venus mRNA was co-injected with either Irs2 MO or control MO into one- to two-cell stage zebrafish embryos. The embryos were raised to 22 h post fertilization and representative embryos are shown. Scale bar, 500 μm. (b,c) Values of body length and somite number are plotted. Blue lines indicate the means±s.e.m. (n=21–34). *Significant difference from the control group (P<0.05, one-way analysis of variance followed by Tukey–Kramer test). (d) Working model on how Nedd4 enhances IGF signalling and mitogenic activity (a detailed explanation is given in the Discussion).
