Figure 3: Modulation of YAP/TAZ activity is accompanied by changes in ciliogenic activity.

(a) Immunofluorescence images showing the nuclear enrichment of YAP/TAZ and concurrent inhibition of ciliogenesis by lysophosphatidic acid (LPA; 10 μM) treatment (24 h) in RPE1 cells, which were serum starved for 24 h. (b) Line graph showing the suppression of ciliogenesis and cilium elongation by LPA treatment. Confluent cells were serum-starved for 24 h with or without LPA treatment at the indicated concentrations. (c) Immunoblot analyses for YAP and phospho-YAP (Ser127) in cells, which were harvested in subconfluence at the indicated times after serum removal. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (d) Subconfluent (50–75%) RPE1 cells were treated with a ROCK inhibitor, Y27632 (25 μM) for 24 h in the presence of 10% FBS. (e) Quantification of experiments illustrated in d. All error bars are s.d. (n=3 independent experiments). All scale bars are 20 μm.