Figure 5: LATS1/2 is dispensable for serum starvation-mediated ciliogenesis.

(a) Immunoblotting to confirm siRNA-mediated knockdown of LATS1 and LATS2. RPE1 cells were transfected with the indicated siRNAs for 72 h. (b) Quantification of ciliogenesis in cells transfected with the LATS1, LATS2 and LATS1/2 mix siRNAs for 72 h. Cells were serum-starved for 24 h before fixation. (c) Quantification of the subcellular localization of YAP/TAZ in cells double-transfected with LATS1 and LATS2 siRNAs. (d,e) qRT–PCR analyses of the expression of AURKA and PLK1 in cells co-transfected with LATS1 and LATS2 siRNAs. GAPDH was used as a normalization control. All error bars are s.d. (n=3 independent experiments). The P-values were determined by unpaired Student’s t-test; *P<0.05, **P<0.01.