Figure 2: The effect of inducible Clec16a KD in murine B cells.

(a) Percentage of B cells (CD19⁺ cells) from splenocytes of inducible Clec16a KD mice after tamoxifen treatment and the littermates treated with oil as a control in two independent experiments by fluorescence-activated cell sorting analysis. Data from the two experiments were combined. Black and red dots represent data from control (Cntr) and Clec16a KD mice, respectively. The black lines through the dots represent the mean level among the ten control mice and nine Clec16a KD mice, respectively. Two-sided T-test was used to compare the percentage of CD19⁺ cells between Clec16A KD mice and control littermates. (b) Ig production from the supernatants of B cells, purified from Clec16A KD mice splenocytes, cultured with anti-mouse CD40 (100 ng ml⁻¹) for 6 days. Data from two independent experiments were combined. Data are mean±s.d. of seven mice in each group. Two-sided T-test was used to compare the level of each Ig subtype produced from Clec16A KD and control littermates.