Figure 1: Non-uniformity in genome coverage and its impact on the sequencing yield.
From: Calibrating genomic and allelic coverage bias in single-cell sequencing
(a) Dependence of the information yield on the sequencing depth. Deeper sequencing of bulk libraries yields information on a larger population of cells; deeper sequencing of whole-genome amplified single-cell libraries reveals information on a larger fraction of the genome (thick lines). (b) Genome coverage bias at different levels. ‘Amplification bias’ (top): whole-genome amplification generates coverage bias at the amplicon level, which is ∼10–50 kb for multi-strand displacement amplification. ‘Sequencing bias’ (bottom): non-uniformity in the selection of sequencing fragments can be caused by multiple sources of bias including whole-genome amplification: the variation in sequencing coverage can be observed from 100 bp to multiple megabases. (c) Schematic representations of recurrent and random amplification bias from multiple independent amplifications of the same DNA material.
