Table 1 Weighted decay time constant for desensitization (τW) and extent of desensitization (% Des) of wild-type and mutant GABAA and glycine receptor constructs.
From: The desensitization gate of inhibitory Cys-loop receptors
Construct | τ W (s) | % Des | n | Construct | τ W (s) | % Des | n |
|---|---|---|---|---|---|---|---|
α1β2 wt | 17.4±3.6 | 86.4±2.6 | 17 | α1V251Iβ2S247I | 2.5±0.8 | 97.5±0.8 | 5 |
ρ1 wt | 23.3±7.9 | 19±13 | 13 | α1V251Dβ2S247D | 1.3±0.4 | 93.5±1.2 | 4 |
α1V251Fβ2 | 0.78±0.20 | 99.0±0.4 | 6 | ||||
ρ1Δ260-α1 | 8.1±0.5 | 90.7±0.5 | 3 | α1β2S247F | 0.75±0.06 | 97.4±0.5 | 5 |
ρ1Δ346-α1 | 0.20±0.04 | 93.6±2.4 | 6 | α1N307Qβ2N303Q | 11.4±1.0 | 90.8±0.4 | 4 |
ρ1Δ433-α1 | 28.2±2.6 | 11±4 | 3 | α1N307Sβ2N303S | 1.2±0.4 | 97±0.9 | 4 |
ρ1α1(Δ6-postM3) | 1.0±0.4 | 72.2±4.2 | 9 | α1N307Vβ2N303V | 0.19±0.03 | 99.6±0.1 | 7 |
ρ1T349K | 5.0±3.7 | 58.6±8.0 | 9 | α1N307Dβ2 | 4.4±0.1 | 97.9±0.4 | 4 |
ρ1β2(M1–M2 link)+α1(Δ6-postM3) | 10.5±3.3 | 25.3±8.6 | 10 | α1β2N303D | 0.41±0.12 | 87.1±4.1 | 4 |
α1EXT-ρ1TM+INT β2EXT-ρ1TM+INT | 22.9±4.0 | 24.3±10.9 | 4 | α1β2γ2L wt | 15.5±3.2 | 79.8±4.5 | 8 |
α1β2ρ1(M1–M2 link) | 0.33±0.11 | 98.9±0.2 | 11 | α1β2γ2LV262F | 1.3±0.2 | 98.7±0.2 | 7 |
α1(Δ8-postM3)β2ρ1(Δ8-postM3) | 5.8±1.3 | 96.1±1.1 | 6 | α1β2γ2LH318V | 2.2±0.3 | 96.3±0.5 | 5 |
α1(Δ8-postM3) β2ρ1(M1–M2 link+Δ8-postM3) | 4.6±1.5 | 80.4±6.2 | 7 | GlyRα1 wt | 10.7±3.4 | 72.4±8.1 | 12 |
α1ρ1 M3β2ρ1 M3 | 17.4±6.1 | 33.7±10.8 | 6 | GlyRα1ρ1(Δ6-postM3) | 0.88±0.23 | 98.2±1.0 | 6 |
α1L300Vβ2L296V | 26.7±12.7 | 25.6±5.8 | 11 | GlyRα1L298V | 17.2±6.7 | 64.8±6.2 | 3 |
α1G258Aβ2G254A | 4.9±1.4 | 93.5±1.5 | 12 | GlyRα1G256V | 0.84±0.16 | 99.6±0.3 | 9 |
α1G258Vβ2G254V | 1.3±0.6 | 93.3±1.2 | 8 | GlyRα1V294L | 17.1±6.0 | 14.8±8.1 | 7 |
α1V296Lβ2V292L | 93±15 | 30.4±3.3 | 5 | GlyRα1A249F | 0.85±0.65 | 98.0±0.9 | 4 |
α1V251Aβ2S247A | 2.5±0.5 | 96.8±0.6 | 5 | GlyRα1N305V | 0.18±0.05 | 98.5±1.6 | 5 |
