Figure 3: Unbinding force-distance curves show that AKR2A simultaneously binds to ribosome and nascent chain of RNC-OEP7:GFP(90) during translation. | Nature Communications

Figure 3: Unbinding force-distance curves show that AKR2A simultaneously binds to ribosome and nascent chain of RNC-OEP7:GFP(90) during translation.

From: Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation

Figure 3

(a) Schematic for measuring the interaction between AKR2A and RNC-OEP7:GFP(90). (b) Force histograms and representative force-distance curves. Histograms were obtained from the whole set of specific curves for the interaction between AKR2A and RNC-OEP7:GFP(90) (n=1,121). The value indicated by an arrow is the probability of no unbinding events. Gaussian fitting gave the most probable unbinding force. Inset confirms OEP7:GFP(90) translation by SDS–PAGE and autoradiography.

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