Figure 1: β-Actin-GFP incorporation into mature IHC stereocilia is limited to a distal tip compartment. | Nature Communications

Figure 1: β-Actin-GFP incorporation into mature IHC stereocilia is limited to a distal tip compartment.

From: Length regulation of mechanosensitive stereocilia depends on very slow actin dynamics and filament-severing proteins

Figure 1

Transgenic β-actin-GFP expression was induced in mice at age P21–P25. Localization was assessed (a) 1 week, (b) 2 weeks, (c) 20 weeks or (d) 40 weeks after expression was initiated. β-Actin-GFP (green) localized to the tips of the tallest (row 1) stereocilia (arrow in a) and the tips of row 2 stereocilia (arrowhead in a), as well as to cell–cell contacts and the apical surface of the cell. F-actin is stained with phalloidin (red). (e) β-Actin-GFP fluorescence intensity was measured along the length of row 1 stereocilia from the tip towards the base and normalized to background fluorescence (n=76, 423, 118 and 134 stereocilia from at least three mice at 1, 2, 20 and 40 weeks, respectively. Error bars represent s.e.m.). Incorporation increased with time in the ∼0.5-μm region at stereocilia tips, while remaining relatively constant in stereocilia shafts. Scale bars, 5 μm.

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