Figure 5: ADF and AIP1 regulate the stereocilia morphology. | Nature Communications

Figure 5: ADF and AIP1 regulate the stereocilia morphology.

From: Length regulation of mechanosensitive stereocilia depends on very slow actin dynamics and filament-severing proteins

Figure 5

(a–i) Scanning electron microscopy of control and mutant OHC and IHC stereocilia. (a–c) Control hair bundles. (d–f) AIP1 mutant. (g–i) ADF null. In each set, the left panel shows OHCs at lower magnification, the middle shows an OHC and the right shows an IHC at high magnification. AIP1 mutant and ADF null stereocilia in rows 2 and 3 were shorter, irregular in length or missing, while stereocilia in the tallest row were unaffected. Scale bars, 1 μm. (j) The number of stereocilia in rows 2 and 3 was reduced in OHCs from the AIP1 mutant and ADF null (20–30 cells from at least three mice). (k) OHC row 3 stereocilia from the AIP1 mutant and ADF null were shorter than control (500–800 stereocilia from at least three mice). (**P<0.001, analysis of variance). NS, not significant.

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