Figure 1: Determining bulk urea m‡-values. | Nature Communications

Figure 1: Determining bulk urea m‡-values.

From: Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein

Figure 1

Bulk kinetic chevron plots for src SH3 plotting the natural logarithm of the observed relaxation rate as a function of (a) urea concentration or (b) GdmCl concentration in buffer containing 0 M (black), 1 M (blue) or 2 M urea (red). Error bars represent the s.d. of the rate measurement. These data were collected using R19C/N59C src SH3 (the unzipping variant); previous data show that m‡ for this variant is the same as m‡ for wild-type src SH3 and A7C/N59C src SH3 (the shearing variant)31.

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