Figure 3: Determining urea m‡-values under shearing forces. | Nature Communications

Figure 3: Determining urea m‡-values under shearing forces.

From: Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein

Figure 3

(a) The natural logarithm of the unfolding rate (s−1) for A7C/N59C src SH3 plotted as a function of force in buffer containing 0 M (black) and 1 M urea (blue). Data were obtained using force-jump experiments in the optical tweezers and fit using the global analysis described in Methods. Each point represents the weighted average of rate measurements from at least six different protein tethers and error bars represent the s.e. Inset shows the geometry along which shearing forces were applied. (b) Percentage flux through pathways S1 (unbroken lines) and S2 (dotted lines) plotted as a function of force in 0 M (black) and 1 M urea (blue). Flux was calculated using equation (3) and the data from Table 1.

Back to article page