Table 1 Results from kinetic analysis of all unfolding pathways.

From: Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein

Experiment

Pathway

Urea m(M−1)

x U (nm)

ln k U 0 pN, 0 M urea

Bulk chevron

B/Z

0.46±0.04

−1.79±0.14

Unzipping

B/Z

0.40±0.05

0.89±0.02

−3.27±0.12

Shearing (low force)

S1

0.71±0.13

0.18±0.12

−5.04±0.42

Shearing (high force)

S2

0.36±0.15

1.52±0.12

−12.72±1.06