Figure 5: Accumulation of radioactive sulfamethazine.
From: Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology
E. coli BL21(DE3)ΔabgTΔpabA cells expressing YdaH show a significant decrease in [3H]-sulfamethazine accumulation when compared with cells carrying the empty vector. When transformed with plasmids expressing the mutant transporters, D180A, N390, W400A, P418A, R426A, D429A and N433A, the levels of intracellular [3H]-sulfamethazine accumulation were much higher than that of cells expressing wild-type YdaH. Error bars depict the s.d. for n=3. ‘*’ indicates values of BL21(DE3)ΔabgTΔpabA/pET15b and BL21(DE3)ΔabgTΔpabA cells expressing the mutant transporters that are significantly higher than that of BL21(DE3)ΔabgTΔpabA/pET15bΩydaH expressing wild-type YdaH (P<0.001; Student’s t-test).
