Figure 3: Smurf1 maintains the protein synthesis requirement in Sema3A-induced growth cone collapse.
From: Coupled local translation and degradation regulate growth cone collapse
(a) Smurf1 accounts for the protein synthesis dependence of Sema3A-induced growth cone collapse. Rat DRG neurons were transduced upon plating with lentiviral constructs expressing two distinct Smurf1 shRNAs or a control non-targeting shRNA (shLacZ) and grown for 5 DIV. Application of anisomycin (Aniso, 40 μM) reduced Sema3A-induced growth cone collapse in neurons expressing shLacZ but not in Smurf1 knockdown neurons. **P<0.01, one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test, n≥58 growth cones per condition from at least three independent experiments. (b) Overexpression of a catalytically inactive mutant form of Smurf1 (Smurf1 C699A) leads to a Sema3A-induced growth cone collapse that is protein synthesis independent. GST, Glutathione S-Transferase. *P<0.05, **P<0.01, one-way ANOVA with Tukey’s multiple comparison test, n≥53 growth cones per condition from at least three independent experiments. NS, not significant.
