Figure 7: Noxa and Bim synergize in promoting mitotic death in A549 cells.
From: The NOXA–MCL1–BIM axis defines lifespan on extended mitotic arrest
(a) A549 cells were transfected with the indicated siRNAs, synchronized with double-thymidine block and released into medium containing Nocodazole. Cells were harvested after increasing times of mitotic arrest and processed for immunoblotting with the indicated antibodies. (b,c) A549-H2B-mRFP cells were transfected with the indicated siRNAs, synchronized, subjected to treatment with DMSO, Nocodazole or Nocodazole and ABT-737 and analysed by live cell imaging. (b) Box (interquartile range) and whisker (min to max) plots showing the elapsed time (h) between nuclear envelope breakdown (NEBD) and cell death for individual cells. **P<0.01; ***P<0.001 using Mann–Whitney test. The fraction (X/50 events) of cells undergoing the fate of interest is indicated. (c) Fate profiles of individual cells from time is indicated in hours.
