Figure 1: LRP6 and Frz8 interact with each other independently of Wnt ligand.
From: LRP5/6 directly bind to Frizzled and prevent Frizzled-regulated tumour metastasis
(a) Schematic diagram of LRP6 deletion mutants. (b–i) HEK293 cells were transfected with indicated plasmids and harvested for IP/WB analysis after 48 h. IP/WB analysis showed that V5-Frz8 interacted with myc-LRP6, which was not affected by the presence of myc-Frz8-CRD (myc-CRD) (b). myc-LRP6 did not bind to IgG-Frz8-CRD that bound to myc-Wnt3a (c). V5-Frz8 bound to myc-LRP6 (d,e) but not to Flag-Notch (d,e) or myc-LRP6-ΔN (e). IP/WB analysis also showed the interaction of V5-Frz8 with myc-E1-2 (f), myc-E3-4 (g) or myc-LDLR (h), and the interaction of myc-E1-2, myc-E3-4 or myc-LDLR with V5-Frz8 (i). (j) FAR-WB analysis showed the direct interaction of purified myc-LRP6N with V5-Frz8.
