Figure 5: Isl1 is hypermethylated in mammary tumours.
From: DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis
(a) Genome-scale distribution of DMRs identified in mammospheres of normal MaSCs and tumorospheres of tumour-initiating cells prepared from MMTV-Neu-Tg mice (n=3 mice in each). (b) Cluster analysis of top 100 hypermethylated and few hypomethylated genes. (c) Genome browser shot showing chromatin immunoprecipitation sequencing (ChIP-Seq) tracks for H3K27me3 (red) and H3K4me3 (green) levels near Isl1 in embryonic stem cells (ESCs), MaSCs and LPs. (d) UCSC genome browser screenshot showing DNA methylation profile at ISL1 locus in mammospheres and tumorospheres. Red and green colors indicate methylated and unmethylated CpG sites, respectively.
