Figure 3: Mbd2 regulates DC antigen uptake and interaction with CD4+ T cells. | Nature Communications

Figure 3: Mbd2 regulates DC antigen uptake and interaction with CD4+ T cells.

From: A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells

Figure 3

(a,b) WT (blue) or Mbd2−/− (red) BMDCs were cultured for 4 days with CFSE-labelled OT-II T cells in the presence of OVA peptide (a) or protein (b). T-cell proliferation was assessed by flow cytometric analysis of CFSE dilution (six wells per group, one of five experiments). (c) Adhesion of WT CD4+ T cells to WT or Mbd2−/− BMDCs under shear flow conditions (n=mean of T-cell adhesion combined from three separate experiments). (d) WT (blue) or Mbd2−/− (red) BMDCs were incubated with DQ-OVA at 37 or 4 °C, and uptake assessed by flow cytometry (five wells per group, one of more than five experiments). **P<0.01, ***P<0.001, ****P<0.0001 (Student’s t-test (a,b) or ANOVA (c,d). gMFI, geometric mean fluorescence intensity.

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