Figure 4: Mbd2 is vital for optimal Th2 but not for Th1 induction in vivo. | Nature Communications

Figure 4: Mbd2 is vital for optimal Th2 but not for Th1 induction in vivo.

From: A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells

Figure 4

(a,b) WT (grey) or Mbd2−/− (black) mice were injected s.c. with PBS, S. mansoni eggs (a) or heat-killed S. typhimurium (St) (b). Seven days later the draining pLNs were harvested, cells restimulated for 72 h with SEA or St and cytokine secretion assessed by ELISA (five mice per group, one of two to four experiments). (c,d) Mbd2ΔDC (black) mice or littermate controls (grey) were injected s.c. with PBS, S. mansoni eggs (c) or heat-killed S. typhimurium (St) (d). Seven days later the draining pLNs were harvested, cells restimulated for 72 h with SEA or St and cytokine secretion assessed by ELISA (five to six mice per group, one of two to three experiments). Bar graphs show mean+s.e.m. *P<0.05, **P<0.01 (Student’s t-test). pLNs, popliteal lymph nodes; s.c., subcutaneously.

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