Figure 6: DC expression of Mbd2 is required for induction of pulmonary allergic inflammation.

(a) WT mice were sensitized by intranasal administration of WT (blue or grey in b–f) or Mbd2^−/− (red or black in b–f) BMDCs that had been cultured overnight in medium alone (M) or with HDM, then intranasally challenged with 5 μg HDM on days 14 and 15 post BMDC transfer and tissues harvested on day 17. (b) BAL fluid cells were counted, cytospin preparations stained and 200 cells per slide counted for specific cell types. (c) Lung tissue cells were isolated and eosinophilia, neutrophilia and monocyte infiltration assessed by flow cytometry. (d) Cytokines in BAL fluid were measured by ELISA. (e) Lung tissue mRNA expression was assessed by qPCR (normalized against Hprt, a.u.). (f) Proportions of TCRβ⁺CD4⁺Foxp3⁺ Treg cells (Treg) and activated effector TCRβ⁺CD4⁺Foxp3⁻CD44⁺CD69⁺ T cells (Teff) in lung tissues were assessed by flow cytometry. (g) Representative lung sections from recipients of WT or Mbd2^−/− HDM-BMDCs, stained with hematoxylin and eosin (scale bar, 50 μM). Bar graphs show mean+s.e.m. (three to six mice per group, one of six experiments). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (ANOVA). a.u., arbitrary units.