Figure 3: Episome replication in T. pseudonana.
From: Designer diatom episomes delivered by bacterial conjugation
(a) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. (b,c) Images of T. pseudonana wild type (b) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome (c). Scale bar, 2.5 μm.
