Figure 6: Sca1+ SP cells derive from Mesp1-, Nkx2-5-, Isl1-, Gata5- and Wt1-fated cells.
From: PDGFRα demarcates the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium
(a) Cre drivers were crossed with the Ai14 R26R-tdTom line. Left, longitudinal sections of the heart at low magnification. Right, ventricular myocardium and brain at high magnification. Orange, Cre-dependent expression of tdTom; blue, 4′,6-diamidino-2-phenylindole. Scale bar, 1 mm (left), 25 μm (right). (b,c) Quantitation of cardiac SP and non-SP cells derived from the Cre+ ancestors shown. (b) Left, flow cytometry showing no interference of Cre with SP cell number. Right, tdTom in the SP and non-SP fractions of Lin−/Sca1+ cells. (c) Bar graph, % tdTom+ cells; n=3–8; *P≤0.05; **P≤0.001. Data are shown as the mean±s.e.m. for independent experiments.
