Figure 1: Time-course analyses of Gli1 expression in the ovary. | Nature Communications

Figure 1: Time-course analyses of Gli1 expression in the ovary.

From: Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells

Figure 1

(a–j) Expression of Gli1-LacZ in the ovaries was detected by whole-mount β-galactosidase staining (a–d) or fluorescent immunohistochemistry for Gli1-positive cell marker LacZ, granulosa cell marker FOXL2, steroidogenic cell marker 3βHSD and nuclear counterstain DAPI (4,6-diamidino-2-phenylindole) at E17.5 (a, e), E18.5 (b, f), P2 (c, g), P6 (d, h) and 2 months (i, j). The insets are higher magnification of the outlined areas in e–h and j. Arrow, mesonephric tubules; E, embryonic day; Ov, ovary; Ms, mesonephros; P, postnatal day. n=3–5 for each Gli1-LacZ specimen. Scale bar: a–d, 500 μm; e–j, 25 μm..

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