Figure 2: Lineage-tracing experiments for the Gli1-positive cells in the ovary and mesonephros. | Nature Communications

Figure 2: Lineage-tracing experiments for the Gli1-positive cells in the ovary and mesonephros.

From: Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells

Figure 2

(a–n) Lineage-tracing of the Gli1-positive cells in the Gli1-CreERT2; Rosa-LSL-tdTomato embryos was induced by tamoxifen (TM) administration at E12.5 (mesonephros-derived, a–i), or at P2 (neonatal ovary-derived, j–n). The ovaries were examined at different stages of development for tdTomato, steroidogenic marker 3βHSD and 4,6-diamidino-2-phenylindole (DAPI). The staining for 3βHSD was observed not only in theca cell layer but also in granulosa cells in large antral follicles. f–i and k–n are higher magnification images of the outlined areas in e and j in single fluorescence channels. n=3–6 for each Gli1-CreERT2; Rosa-LSL-tdTomato specimen. Scale bar: a–d, 500 μm; e and i, 25 μm.

Back to article page