Figure 4: Dhh and Ihh in the granulosa cells are responsible for Gli1 expression in the theca progenitor cells. | Nature Communications

Figure 4: Dhh and Ihh in the granulosa cells are responsible for Gli1 expression in the theca progenitor cells.

From: Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells

Figure 4

(a–c) Effects of the Hedgehog inhibitor cyclopamine on expression of Gli1-LacZ in the ovary. Gli1-LacZ ovaries (E18.5) were cultured in the presence or absence of cyclopamine (25 μM) for 3 days followed by LacZ staining (blue). Ov=ovary; Ms=mesonephros. n=5–6 for each specimen. Scale bar, 500 μm. (d) Quantitative PCR (qPCR) analysis of Gli1, Ihh and Dhh mRNA expression in E17.5 (n=5) and P3 (n=5) ovaries. (e) qPCR analysis of Gli1, Ptch1, Ihh and Dhh mRNA expression in granulosa cells (n=5) and theca progenitor cells (n=3). Cells isolated from a pair of ovaries were pooled as n of 1 (see Supplementary Fig. 8 for details). **P<0.01; ***P<0.001; ****P<0.0001; Two-tailed Student’s t-test. Values in all graphs are presented as means±s.e.m.

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