Figure 7: Temperature unfolding of conformational switches.
From: Design of protein switches based on an ensemble model of allostery
(a) Temperature unfolding transitions of c4 (solid red), c4 with 5 mM maltose (solid blue), c4-3G (dotted pink) and c4-3G with 5 mM maltose (dotted light blue) as assessed by the far-ultraviolet CD spectra at wavelength 222 nm. Open circles represent relative enzymatic activity of c4 as a function of temperature in the absence of maltose. Temperature unfolding transitions of (b) c4 and (c) c4-3hx in pH 6 (dotted green), pH 6 with 5 mM maltose (solid green), pH 8 (dotted dark green) and pH 8 with 5 mM maltose (solid dark green) as assessed by the far-ultraviolet CD spectra at wavelength 222 nm. Open circles represent relative enzymatic activity of c4-3hx as a function of temperature in the absence of maltose.
