Figure 5: Effect of cholesterol depletion and temperature change on CD59 mobility.
From: GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane
(a) Relative diffusion coefficients DON/DOFF of CD59 as a function of average mGFP-GPI spacing dmGFP-GPI (open diamonds) and after treatment with 1 U ml−1 cholesterol oxidase (closed diamonds; N=34, six independent experiments). (b) Relative diffusion coefficients DON/DOFF of CD59 as a function of average mGFP-GPI spacing dmGFP-GPI at 23 °C (open diamonds) and at 37 °C (closed diamonds; N=11, three independent experiments).
