Figure 4: Prdx4 inhibits GDE2 activity by targeting its extracellular Cys residues. | Nature Communications

Figure 4: Prdx4 inhibits GDE2 activity by targeting its extracellular Cys residues.

From: Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2

Figure 4

(a) Representative western blot (one of three individual experiments) shows co-IP of Prdx4 and GDE2 using extracts from transfected HEK293T cells. (b–d) Close-up of ventral regions of chick spinal cords that are electroporated on the right side. VZ, ventricular zone; arrows mark midline. Scale bars, 20 μm. Graphs quantifying number of Isl1/2+ cells (e) or percentage of LacZ+ cells that are Isl1/2+ (f–i) are induced in VZ progenitors. GDE2 expressing constructs were bicistronic for LacZ; LacZ was used as a measure of electroporation efficiency. Mean±s.e.m., schematics on the right show location of Cys residues (black circles) in GDE2, red circles are Cys residues that were mutated to Ser. 0.5 × GDE2 was used in f and g as these versions of GDE2 are hyperactive (e) GDE2+Prdx4 *P=3.7864E-05; GDE2+Prdx4C118.239S P=0.1519; n=4–7 embryos; (f) 0.5 × GDE2C25.576S+Prdx4 *P=0.0009, n=9–12 embryos; (g) 0.5 × GDE2C25S+Prdx4 *P=0.0425, n=5–10 embryos; (h) GDE2C15.18S+Prdx4 *P=0.0003, n=8–9 embryos; (i) GDE2C340S+Prdx4 P=0.7888, n=5–6 embryos, two-tailed Student’s t-test.

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