Figure 5: Prdx4 oxidizes Cys residues within the GDE2 GDPD domain. | Nature Communications

Figure 5: Prdx4 oxidizes Cys residues within the GDE2 GDPD domain.

From: Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2

Figure 5

(a,b,d,e) Western blots of biotin labelling assays to measure thiol oxidation. (c–e) Graph quantifying biotin incorporation normalized to total GDE2, GDE2C340S or GDE2C467S. Mean±s.e.m., n=8–13 different transfection experiments. Compared with GFP (green fluorescent protein) controls, (c) Prdx4 *P=0.0159, Prdx4C118.239S P=0.6955; (d) Prdx4 P=0.3022, Prdx4C118.239S P=0.0854; (e) Prdx4 P=0.9249, Prdx4C118.239S P=0.4988, two-tailed Student’s t-test. (f) Sequence of the GDE2 GDPD domain located between transmembrane (TM) regions 5 and 6. Sequences in yellow denote peptides identified by mass spectrometry; C340 and C467 are shown in red.

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