Figure 6: GDE2 surface expression in motor neurons is redox sensitive and Prdx4 dependent. | Nature Communications

Figure 6: GDE2 surface expression in motor neurons is redox sensitive and Prdx4 dependent.

From: Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2

Figure 6

(a–c) Western blots of surface biotinylation assays using membrane fractions of primary motor neuron cultures derived from E11.5 spinal cords. In c, arrow marks detection of band corresponding to GDE2, which is absent in Gde2 null animals. Graphs show surface levels of biotinylated GDE2 normalized to total levels of membrane GDE2. Mean±s.e.m., (a) *P=0.003, n=14 separate cultures; (b) Tempol *P=0.021, Edaravone *P=0.006, n=11 separate cultures; (c) *P=0.035, n=9–24 embryos; two-tailed Student’s t-test.

Back to article page