Figure 1: The dHP1c complex interacts with dDsk2.

(a) dDsk2 co-immunoprecipitates with components of the dHP1c complex. On the top, IPs were performed with rat polyclonal αdHP1c (lane 3), αROW (lane 4) and control αDDP1 (lane 2) antibodies. On the bottom, IPs were performed with rabbit polyclonal αWOC (lane 3), αROW (lane 4) and control αDDP1 (lane 2). IP materials were analysed by western blot analysis using rabbit polyclonal αdDsk2. Lane 1 corresponds to 2.5% of the input material. At the bottom, the asterisk (*) indicates a band corresponding to IgGs. The position of markers of known molecular weight (kDa) is indicated. (b) Components of the dHP1c complex co-immunoprecipitate with dDsk2. IPs were performed with rabbit αdDsk2 (lane 4), and control αWOC (lane 3) and αDDP1 (lane 2) antibodies, and analysed with western blot analysis using rat polyclonal αROW (top) and αdHP1c (bottom). Lane 1 corresponds to 2.5% of the input material. The position of markers of known molecular weight (kDa) is indicated. (c) Glycerol gradient (10–30%) sedimentation analysis of a total S2-cell protein extract. Fractions were collected from top to bottom and were analysed with western blot analysis using rat polyclonal αdHP1c, αdHP1b and αROW, rabbit polyclonal αdDsk2 and mouse polyclonal αp54/Rpn10. Fractions 2–34 are shown. Lane 1 corresponds to 2.5% of the input material. The position of markers of known molecular weight (kDa) is indicated. (d) dHP1c and dDsk2 strongly colocalize. Polytene chromosomes were immunostained with rat polyclonal αdHP1c (green) and rabbit polyclonal αdDsk2 (red) antibodies. Enlarged images of the indicated region are shown at the bottom. DNA was stained with DAPI (blue). Scale bar, 10 μm.