Figure 3: dHP1c complex target genes are enriched in developmental functions and show strong RNA pol II pausing.
From: dDsk2 regulates H2Bub1 and RNA polymerase II pausing at dHP1c complex target genes
(a) The percentages of dHP1c complex target (red) and non-target genes (green) are shown for the 10 most enriched functions. Statistical significance of the differences (Benjamini–Yekutieli-adjusted Fisher exact test P value) is indicated. (b) On the top, the coverage profiles across a representative region are presented for total RNA pol II (RPB1), short RNAs and KMnO4 reactivity. Genomic organization of the region is indicated. On the bottom, the distribution of total RNA pol II (RPB1) across a standardized gene (left) and a box plot showing the RNA pol II pausing index (right) are presented for dHP1c complex target (red) and non-target (black) genes. (c) Venn diagrams showing the intersection between dHP1c complex target genes and Pol IIoser5 (left), NELF-E (centre) and short RNA (right) target genes. (d) On the left, a Venn diagram showing the intersection between dHP1c complex target genes and genes showing significant KMnO4 reactivity is presented. On the right, the percentage of dHP1c complex target genes showing low (min to first quartile; white), medium (first to third quartile; light green) and high (third quartile to max; dark green) KMnO4 reactivity is compared with NELF-E and Pol IIoser5 target genes, and all genes.
