Figure 2: Macrophage IL-10 regulates myeloid cell recruitment to the colon. | Nature Communications

Figure 2: Macrophage IL-10 regulates myeloid cell recruitment to the colon.

From: IL-10-producing intestinal macrophages prevent excessive antibacterial innate immunity by limiting IL-23 synthesis

Figure 2

Il10flox/floxLysM-Cre (black), Il10flox/floxCd4-Cre (white) and Il10flox/flox (grey) mice were analysed before and at day 6 after C. rodentium infection. (a) Lamina propria cells were isolated at the indicated times and total cell numbers were counted. Frequencies of CD4+ T cells (CD4+TCRβ+), monocytes (Ly6G−Ly6Chigh) and granulocytes (Ly6GhighLy6Clow) were measured by flow cytometry and cell numbers were calculated according to the respective total cell number. Cell numbers are presented as averages and s.d. from nine mice per group from three independent experiments. (b,c) At the indicated time points, 3-mm fragments of the middle colon of Il10flox/floxLysM-Cre (black), Il10flox/floxCd4-Cre (white) mice were cultured for 24 h. Chemokine production was measured in the supernatants by Multiplex cytokine assay and normalized to the weight of the corresponding tissue fragments. Data are presented as averages with s.d. from two independent experiments with six mice in each group. Student’s t-test, *P<0.05, **P<0.01, ***P<0.001.

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