Figure 5: Stochastic development of the circadian system in ClkSV40 flies
From: Clk post-transcriptional control denoises circadian transcription both temporally and spatially
(a) Number of PDF-positive cell bodies in ClkWT and ClkSV40 brains. Data are from the ClkSV40 (2–8) and ClkWT (1–1) fly strains. (b) Representative example of a ClkSV40 fly brain in which the position of extra pdf-expressing cells can be visualized. (c) Representative example of a ClkSV40 fly brain in which 14 pdf->expressing cells were observed in one of the brain hemispheres. Z-stack of three images (each taken every 1 μm). Numbers in the upper left corner of each picture indicate the relative position of the Z-stack. Arrows indicate the position of the pdf->expressing cells. (d) Z-stack (maximal projection) of two brain hemispheres of ClkSV40 flies immunostained for PDF showing the number of pdf-expressing cells (13 and 12 for the top and bottom brain hemispheres respectively). Arrows indicate the position of the pdf->expressing cells. Red scale bar indicates 10 μm. (e) Z-stack (maximal projection) of two brains of ClkSV40 pupae immunostained for PDF (in red) and VRI (in green) showing the number of pdf-expressing cells (14 and 15 for the left and right images respectively). Arrows indicate the position of the pdf->expressing cells. Red scale bar indicates 10 μm. (f) Comparison of left and right hemispheres in ClkWT and ClkSV40 flies based on PDF immunofluorescent staining. (g) Brain-to-brain variation in the levels of VRI (measured by IF) protein in the sLNvs at 4 time points (day 10 in DD), in ClkSV40 (line 2–8, red) and ClkWT (line 1–1, black) flies. See also Supplementary Figs 5–8 Supplementary Movies 3 and 4.
