Figure 3: Nrf2 is epigenetically suppressed during early neuronal development. | Nature Communications

Figure 3: Nrf2 is epigenetically suppressed during early neuronal development.

From: Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2

Figure 3

(a) Neuronal Nrf2 expression measured at DIV2 and DIV9. *P<0.05, (n=4). (b) Nrf2 mRNA expression analysis in NeuN− versus NeuN+ cells obtained from acutely dissociated P0 mouse cortices by FACS (n=5). (c) Whole cortex Nrf2 expression in P0 pups compared with adult mice. *P<0.05 (n=4 (adult), 6 (P0)). (d) A comparison of NRF2 mRNA expression in neurons and astrocytes derived from hESCs, compared with neural precursor cells (NPCs) and undifferentiated (u/d) hESCs (dashed line). *P<0.05 (n=5 (NPCs), n=3 (neurons and astrocytes)). (e) Acetyl-H3 ChIP analysis of the Nrf2 promoter at DIV2, compared with DIV9. *P<0.05, (n=5). (f) TSA treatment of DIV2 neurons does not increase Nrf2 mRNA expression (n=4). (g) Expression of Nrf2 target genes (analysed by qRT–PCR) compared between DIV2 and DIV9 neurons. *P<0.05 (n=4). (h) Analysis of the indicated Nrf2 target genes in Nrf2+/+ and Nrf2−/− DIV2 neurons treated with vehicle or tBHQ suggests a functional Nrf2 pathway at this stage. *P<0.05 (n=5 (wild type (WT)), 4 (knockout (KO)). (i) Analysis of Cat and Gclc expression in Nrf2+/+ and Nrf2−/− DIV2 neurons. *P<0.05 (n=5 (WT), 4 (KO)). (j) Quantification of H2O2-induced cell death in Nrf2+/+ and Nrf2−/− DIV2 neurons. *P<0.05 (n=4, 1,700–4,500 cells analysed per condition per genotype).

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