Figure 3: Cell cycle-specific oscillation of Ndd1 levels depend on APC/CCdh1 activity. | Nature Communications

Figure 3: Cell cycle-specific oscillation of Ndd1 levels depend on APC/CCdh1 activity.

From: Degradation of Ndd1 by APC/CCdh1 generates a feed forward loop that times mitotic protein accumulation

Figure 3

(a) Cdh1Δ cells expressing gal-inducible Cdc20 were grown to mid-log phase in raffinose. Cells were subsequently arrested by nocodazole for 2 h in mitosis, treated with galactose to induce Cdc20 and sampled at the indicated time points. Cdc20 is as expected strongly induced leading to degradation of Clb2, but not to degradation of Ndd1. Mid-log populations of wild-type (b) or cdh1Δ (c) cells were arrested with alpha factor for two hours, released and sampled at 10-min intervals.

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