Figure 2: Spontaneous formation of splenic GCs.

(a) Immunofluorescence staining of spleen cryosections from control (Ctrl) and SLC^−/− mice. GL7 (green), B220 (red) and TCRβ (blue). Arrows indicate GC structures, × 10 objective; scale bars, 200 μm. Graph shows % GCs per follicles (FO, two independent observers). (b–e) Flow cytometry analysis of spleen cells. (b) Dot plots show CD95⁺GL7⁺ GC B cells among CD19⁺ B cells. (c) Graph shows % CD95⁺GL7⁺ GC B cells among CD19⁺ B cells in Ctrl and SLC^−/− mice. (d) Graph shows % CD95⁺GL7⁺ cells in CD19⁺ B cells at indicated ages in Ctrl and SLC^−/− mice. (e) Graph shows frequency of mice with ≥1.0% CD95⁺GL7⁺ cells in CD19⁺ B cells at indicated ages in SLC^−/− mice. Data in a and b are representative, and in a and c–e are pooled from at least two independent experiments. Each symbol represents one mouse. Error bars represent s.e.m. P values were determined using a two-tailed t-test. *P<0.05; **P<0.01; ***P<0.001; NS, not significant; w, weeks.