Figure 3: Gut microbiota is not a main trigger of spontaneous GC formation. | Nature Communications

Figure 3: Gut microbiota is not a main trigger of spontaneous GC formation.

From: Absence of surrogate light chain results in spontaneous autoreactive germinal centres expanding VH81X-expressing B cells

Figure 3

(a–g) SLC−/− mice were treated with broad-spectrum antibiotics or saline water. (a) Experimental design: antibiotic treatment of SLC−/− mice was started at an age of 3–4 weeks (w) and completed 4 weeks later. (b) Dot plots show CD95+GL7+ cells in CD19+ cells from Peyer’s patches (PP). (c) Graph shows % of CD95+GL7+ cells in CD19+ cells from PP. (d) Immunofluorescence staining of intestine cryosections with antibodies detecting IgA, × 10 objective; scale bars, 50 μm. (e) qPCR analysis of total bacterial DNA copy numbers in faeces. (f) Graph shows total splenic CD19+ cell numbers. (g) Graph shows % splenic CD95+GL7+ cells among CD19+ cells. Data are representative from two independent experiments. Each symbol represents one mouse. Error bars represent s.e.m. P values were determined using a two-tailed t-test. **P<0.01, ***P<0.001; NS, not significant.

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